By Edmund T. Whittaker
The aim of this quantity is to explain the revolution in physics which came about within the first zone of the 20th century, and which integrated the discoveries of particular Relativity, the older Quantum concept, common Relativity, Matrix Mechanics and Wave Mechanics.
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Extra info for A history of theories of ether and electricity. The classical theories
Biological particles may be stained with the same ﬂuorochromes used in experiments to stain cells. Examples of data for some of the more common types of particles follow. , 1996, 1998). 2 show emission spectra from three types of particles: ﬂuorochrometagged beads (CaliBRITE beads, BD Biosciences), stained with either ﬂuorescein isothiocyanate (FITC) or phycoerythrin (PE); broad-spectrum hard-dyed beads (Rainbow beads, Spherotech); and glutaraldehyde-ﬁxed chicken red blood cells (gCRBC, BioSure).
1988; Steen, 1992) can be developed based on optical detection efﬁciency, Q (number of photoelectrons per ﬂuorochrome molecule analyzed), and background light, B. B can be expressed in units of the equivalent number of ﬂuorochrome molecules that would produce that background. The studies by Gaucher et al. (1988) and Steen (1992) used ﬂashes from a light-emitting diode (LED) to produce dim signals to a ﬂow cytometer detector and used the CVs of the resulting signals to determine Q. 14 Supplement 32 Current Protocols in Cytometry shining on the sample stream to those obtained with the laser blocked.
CD8) can give different ABC values on the same cells. Whether this is due to differences in binding of the primary antibody to cells or to differences in binding of the secondary antibody to the primary antibody is not clear. Altered reactivity of the second-step reagent with ﬂuorochrome-conjugated mouse monoclonal antibody is another possible variable. , 1996). The QSC method is designed to capture quantitatively any mouse monoclonal antibody independent of ﬂuorochrome conjugation or IgG isotype.
A history of theories of ether and electricity. The classical theories by Edmund T. Whittaker